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For each time point lucifer yellow fluorescence inside and outside the organoids was quantified using Fiji ImageJ

Cells were lysed with 500 L of RIPA lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris-HCl, supplemented with protease and phosphatase inhibitors at a 1:100 dilution

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It is applied in exercise and recovery models, where supplementation has shown potential to support performance, lower lactate levels, and reduce muscle damage markers