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Administration of PLX3397 to mice For pharmacological ablation of microglia, PLX3397 was administrated to mice by supplementation (290 mg/kg of PLX3397 (#C-1271

[28] mhra.gov.uk or via the Yellow Card app), which helps monitor the safety of medicines and supplements in the UK

Clean the vial top again with an alcohol swab, draw the additional water in a fresh syringe, and add it slowly down the glass wall

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For further guidance on aseptic preparation and beyond-use dating of injectable products in UK practice, refer to SPS (Specialist Pharmacy Service) resources and MHRA guidance on the manufacture of IMPs

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