(TIF) Figure S3
After 30 min, wells were aspirated, PBS was added and plates were sealed with adhesive foil (VWR, 60941-124)
After O/N culture, cells were pretreated with intermediates of cholesterol biosynthetic pathway for 3 h, drugs were washed out with serum-free medium, followed by incubation with 5 M BODI1PY -C11 581/591 and RSL3 (250 nM) at 37 C for 2 h in the dark
Diagnostic Challenges and Pathophysiological Correlates Because the clinical and diagnostic aspects are discussed elsewhere, only mechanistic considerations are highlighted here
4 ROS and antioxidants: dual roles in cellular physiology ROS processes that affect GSH homeostasis are summarized here, as this determines the activation threshold of GSH-responsive nanomaterials
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