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Lysates were boiled at 95 C for 5 min, 20 g of total protein were subjected to separation by a 10% SDS gel, transferred onto nitrocellulose membranes (Amersham Biosciences) and blocked with 5% milk powder in PBS Tween (0.1%) for 1 h

EEG findings in LS are variable

The carbon-rich precursors, such as green natural and biomass-derived materials being the most prominent contributors [55], acting as the source of carbon when incorporated with top-down or bottom-up synthesis methods