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Pass/Fail per US Pharmacopeia limits

Fluorescence images were sequentially acquired with a Nikon A1R+ laser scanning confocal Eclipse Ti-E inverted microscope (Nikon Corporation, Tokyo, Japan) equipped with a motorized stage (TI-S-E, Nikon)

PMID: 33497204 )

Rat Acetylcholine (ACh) ELISA Kit, Rat Vasoactive Intestinal Peptide (VIP) ELISA Kit, Rat Gastrin (Gas) ELISA Kit, Rat Motilin (MTL) ELISA Kit, Rat Catalase (CAT) ELISA Kit, Rat Superoxide Dismutase (SOD) ELISA Kit, Rat glutathione peroxidase (GSH-Px) ELISA Kit, Rat Choline Acetyltransferase (ChAT) ELISA Kit were purchased from Gelatins (Jiangxi, China)

Feed samples were dried in the oven and were ground through a 1-mm screen for chemical composition analysis

Additionally, they revealed a 50% improvement in the total ABC scores, a significant difference in the change of plasma transferrin levels and a trend towards a significant difference in plasma superoxide dismutase in the treatment arm