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Slides were treated with HIER buffer (10 mM sodium citrate, 0.05% Tween-20, pH 6.0) for antigen retrieval in a pressure cooker for 10 min then sections were blocked in CAS-block histochemical reagent (Thermo Fisher Scientific, 008120)

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Virus-induced GH overexpression in the amygdala increases the number of amygdala cells activated by fear memory formation and enhances dendritic spine density in GH-overexpressing neurons (73)

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