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Tissue fixation and preparation of paraffin-embedded, frozen, and vibratome-cut sections Brain hemispheres were fixed by immersion in 4% paraformaldehyde in phosphate buffer solution (Nacalai tesque, Kyoto, Japan)

Within the complex, p53 TAD2 residues 47 to 54 displayed increased rigidity upon binding and consistently maintain an -helical conformation throughout the MD calculations (Fig