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The supernatant was then centrifuged at 8,000g and the resulting pellet containing the mitochondria fraction was re-suspended in 100 mM phosphate buffer (pH = 7.4)

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mRNA levels of potential c-Jun target genes were analysed by real-time PCR

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This coordinated release scheme is reminiscent of neuromodulatory D-serine co-transmission at NMDA receptors, and may even suggest co-release of D-serine and polyamines from astrocytes under these conditions (with the caveat that D-serine gliotransmission is controversial) (Figure 2