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Abstract Objective Neurodegenerative diseases are a leading cause of disability worldwide, and recent evidence highlights the role of angiogenesis in their pathophysiology

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CTK solution (0.25% trypsin + 1 mg/mL collagenase IV + 1 mM CaCl 2 + 20% KSR in DPBS ()) was prepared with trypsin ((2.5%), no phenol red, Thermo Fisher Scientific Inc.), collagenase (type IV, powder, Thermo Fisher Scientific Inc.), calcium chloride dihydrate (Fujifilm Wako Pure Chemical Corporation), Knock Out TM Serum Replacement (KSR, Thermo Fisher Scientific Inc.)

The elderly, however, are among the ones that are at risk with this deficiency

This compound is supplied strictly for research purposes, and none of this research constitutes clinical evidence of efficacy in human subjects

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