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Cells were harvested, washed once with phosphate buffered saline (PBS, Gibco, #14190-094) and loosened from the cell flasks with Versene (Gibco, #15040-033) or TrypLE TM (Gibco, #12605-010)

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DIRECTIONS with MTS bottle: Fill the bottle with 1ml of serum, which you can measure with the dropper that comes with the serum

Those are useful, but there are serious limitations regarding how benefits to animals can be translated to benefits to people

In MS-DRG 219, we identified a total of 1,136 cases reporting procedure code combinations describing open concomitant surgical ablations with an average length of stay of 11.2 days and average costs of $70,693