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Primary antibodies to CD44 (Abcam, #ab189524, 1:30.000), GPX4 (Abcam, #ab125066, 1:5000), GR1 (Cell Signaling/New England Biolabs, #12041, 1:1000), and STING (Cell Signaling, #13647S, 1:1000) were diluted in 5% BSA (Serva, 11930.004)

Our study suggests that the increased expression and nuclear distribution of p53-pS15 in keloids are associated with the promotion of pro-senescence regulation causing the cell cycle arrest and resistance to apoptosis

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Following methodical steps improves overall laboratory safety and preparation accuracy

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