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Then the optical density at 600 nm (OD 600 ) was determined, and the appropriate volume of bacterial culture (x L, where x = 200 0.8/OD 600 ) was collected by centrifugation at 4000 rpm for 2 min

Zhou, Busulfan-induced senescence is dependent on ROS production upstream of the MAPK pathway

10.4103/jpn.JPN_130_19 [DOI] [PMC free article] [PubMed] [Google Scholar] Bao Z

Briefly, metabolites in plasma (100 L) were extracted with 90% methanol (900 L), and proteins were precipitated at -20 C for 2 h, followed by centrifugation for 10 min at 4000 rpm at 4 C

Allow up to 5 minutes

Storage: 28C (refrigerated after reconstitution)