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Viability was assessed by Zombie UV Fixable Viability Dye (BioLegend) and cytokine production was measured by intracellular cytokine staining after 3 h stimulation with PMA (50 ng ml 1 ) (BioGems) and ionomycin (1 g ml 1 ) (BioGems) in the presence of brefeldin A (1 g ml 1 ) (GolgiPlug, BD Biosciences)

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The extracted RNA was reverse transcribed into cDNA with HiScript III RT SuperMix for qPCR (R323-01, Vazyme, China), and cDNA was used to perform qRTPCR with the ChamQ Universal SYBR qPCR Master Mix (Q711-02, Vazyme, China)

Telomeres and cardiovascular disease: Does size matter