After the percentage record of linear motile spermatozoa at 200 magnification under a phase contrast microscope in three different microscopic fields for each sample, the final motility ratio of frozen-thawed sperm motility in each group was calculated based on the mean of three successive estimations ( The frozen-thawed sperm viability was analyzed by eosin-nigrosin staining ( Giemsa staining of frozen-thawed spermatozoa (repeated in triplicated) was performed for the acrosome integrity assay ( After incubation with hypo-osmotic swelling solution [HOS, containing 6.75 g/L fructose and 3.67 g/L sodium citrate] at 37C for 1 h, plasma membrane integrity of each group (repeated in triplicated)was analyzed by evaluating the modifications of sperm tail to score swollen sperm according to previous publications ( For mitochondrial function assay, methyl thiazolyl diphenyl-tetrazolium bromide (MTT) assay was performed
And human studies are scarce
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Plant Physiol 87:206210 Wolf AE, Dietz KJ, Schrder P (1996) Degradation of glutathione S-conjugates by a carboxypeptidase in the plant vacuole
doi: 10.1016/j.ebiom.2022.103847 108 WangLHeTLiuJTaiJWangBZhangLet al
