A matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) assay revealed several proteins with antioxidant functions including a GST that were differentially expressed between compatible and incompatible interactions, indicating the differential accumulation of ROS in infected tissues (Li et al., Hemibiotrophic Fungi The important role of GSTs in antifungal plant resistance was demonstrated also in hemibiotrophic plant-fungus interactions
Chuma, S., Hosokawa, M., Tanaka, T
GDNF-transfected macrophages ameliorate symptoms of Parkinsons disease in mice
Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing
It might seem intuitive, albeit incorrect, to calculate GSH levels over the cycle by calculating the steady-state GSH for each day of the cycle
Srinivasan R., Ratiney H., Hammond-Rosenbluth K.E., Pelletier D., Nelson S.J
