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Here is a detailed examination of these challenges and the corresponding solutions: Tag-Induced Protein Aggregation Challenge : One of the primary issues with GST-tagged proteins is that the GST tag can sometimes induce aggregation or interfere with the folding of the target protein

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A Facile one-pot Synthesis of water-soluble CQDs for the Evaluation of Their Anti-amyloidogenic Propensity

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It may also be prescribed when oral supplementation is not possible or not sufficient, for example after certain gastrointestinal surgeries or in severe malabsorption