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The isolated metatarsals were cultured in 24-well culture plates in 150 L of DMEM supplemented with 10% heat-inactivated fetal bovine serum and 1% of penicillin/streptomycin and 1% L -glutamine for 72 h

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Li Y, Luikart BW, Birnbaum S, Chen J, Kwon CH, Kernie SG, et al