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The primary antibodies employed were: P53 (Proteintech, 60283-2-Ig), pSer46-P53 (CST, 2521), FOXO4 (Proteintech, 21535-1-AP), P21 (CST, 2947), P16 (CST, 18769), BAX (CST, 2772), BCL2 (CST, 3498), Ki-67 (Proteintech, 84192-4-RR), Lamin B (CST, 13435), Cleaved Caspase 3 (CST, 9664), -H2AX (Abcam, ab81299), and GAPDH (Abcam, ab8245) as an internal reference for normalizing protein expression levels
the internal chronic inflammatory cells, particularly tgams, continue to release inflammatory mediators and proteases such as interleukin-1 (IL-1 ), tumor necrosis factor - (TNF - ), and matrix metalloproteinases (MMPs)
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If DSIP enhances deep sleep, it could theoretically amplify this natural hormone release window
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