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For a broader look at what B12 does beyond energy including its role in nerves, methylation, and brain health see our complete vitamin B12 benefits guide

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All mice were housed under a 12 h light/dark cycle, with the temperature consistently maintained between 18 C and 23 C and the humidity kept at ~50%

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B12 is generally considered safe in all life stages even at high doses and there are no known interactions with any medications, but some drugs can increase the risk of B12 deficiency, including acid-lowering drugs and metformin

Pharmacol., 21 : 315-330