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The HTR and JEG-3 cells were respectively cultured in RPMI-1640 and MEM Medium (P/S) with 10% fetal bovine serum, at a temperature of 37 C, with 5% CO 2 , and were passaged every two days

Future investigations could benefit from techniques such as CUT&Tag (Cleavage Under Targets and Tagmentation) or CLIP-seq (Cross-Linking Immunoprecipitation followed by Sequencing), particularly m6A-specific variants like m6A-CUT&Tag or miCLIP/m6A-CLIP-seq

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It dissolves under the tongue or against the cheek, absorbing through the oral mucosa rather than passing through the gastrointestinal tract

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