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Glutathione concentrations in the cytoplasm are typically in the range of 1-2mM (although in some cells such as hepatocytes it can be upto 10mM) (Forman et al., 2009)

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Typical Assay Conditions: Aqueous buffer : dH20 + 0.1-2% DMSO Incubation : Up to 24 hours Analysis : LC-MS/MS after centrifugation Compound Requirements : 10 mM DMSO stock solution