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After the first round of refinement, the datasets were subjected to CTF refinement to estimate beam tilt, anisotropic magnification, per-particle defocus and astigmatism, and also Bayesian polishing of beam-induced motion-correction with either default (wt PV2-CP17) or trained (PV3-SC8 GPP3+GSH and PV3-SC8 pleconaril+GSH ) parameters 38