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More significant results might have been obtained if bulk NGS analysis had been performed on brain samples collected during the active phase of weight loss, i.e

doi: 10.1038/ncomms15965 252 ReisLMDAdamoskiDOrnitz Oliveira SouzaRRodrigues AscenoCFSousa De OliveiraKRCorra-da-SilvaFet al

The 10mg format provides sufficient material for multiple experimental runs without frequent reordering, aligns with quantities referenced in the majority of published laboratory research, offers better cost efficiency per milligram for established programmes, and maintains stability in lyophilised storage at -20C across a typical research project

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