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The bacteria are then purified away from cell debris and cytosol by centrifugation through a discontinuous gradient of 60, 30 and 20% (w/v) iodixanol at 45,000 g for 1 h [5]

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doi: 10.2147/JIR.S302929 182 GeGBaiJWangQLiangXTaoHChenHet al