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A triiodothyronine ELISA kit (LifeSpan BioSciences, Seattle, WA, USA) and thyroxine (T4) EIA kit (Arbor Assays, Ann Arbor, MI, USA) were used to detect serum triiodothyronine and thyroxin, respectively, according to the manufacturers instructions

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To assess whether the addition of albumin has any influence on aggregation of CIPp-18, we measured changes in its absorption and fluorescence spectra subsequent to the addition of varying concentrations of BSA