Whether the breadth translates to clinical benefit on intact human skin depends entirely on whether the molecule reaches the cells whose gene expression it modulates
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Immunofluorescence staining Fibroblasts were fixed, permeabilised, blocked, and incubated overnight at 4 C with primary antibodies targeting p53-pS15 and FOXO4 shown in Table 2, and developed using Alexa Fluor 488-conjugated donkey anti-mouse immunoglobulin G (1:100
control doi:10.1371/journal.pone.0162590.t001 Table 2
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