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Nature 496 , 238242 (2013)

Detailed methods to purify a total NPC fraction are described in OptiPrep Application Sheet C26 A total NPC fraction is often a starting point for purification of Kupffer cells and/or endothelial cells by non-density gradient methods (adherence to a substratum or centrifugal elutriation)

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doi: 10.1016/j.ajpath.2024.01.019 384 LiuZGanSFuLXuYWangSZhangGet al

After adding the solvent, gently swirl the vial or roll it between your palms

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