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ChaC-like enzymes act specifically on GSH, GGACT enzymes act on -glutamylamine amino acids, and GGCTs have a broader activity towards -glutamyl amino acids in general

Some practitioners inject near injury site (local administration) versus systemic injection, though evidence for superiority of local injection limited

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Sample sizes (typically 36 biological replicates per group) were selected based on established standards in the field and our preliminary data, which indicated that this range was sufficient to detect biologically relevant effects