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TMT labeling and fractionation Desalted peptides were labeled with TMTpro reagents (Thermo Fisher Scientific)

Stimulation of de novo glutathione synthesis by NFT concentrations in this range was observed in HepG2 cells, PHHs, or human neuronal cells, but not to the same extent in other cell types such as human iPSC-derived astrocytes

To confirm the gene expression data, cell extracts were acquired from MP- and mock-inoculated resistant (SC599, SC35) and susceptible (Tx7000, BTx3042) sorghum genotypes and their reduced (GSH), oxidized (GSSG) glutathione, GST, GPX, and GR activities were measured using standard protocols

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They found that REG1, REG2, and GSHPX1 were differentially expressed in the pancreas of normal mice and mice with diabetes

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