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Proteins were transferred to PVDF membranes (Thermofisher) using a Power BlotterSemi-dry Transfer System (Thermofisher) and incubated overnight with IFN- (E3V1X) Rabbit mAb (Cell Signaling, #98139S), Galectin-1/LGALS1 (D608T) Rabbit mAb (Cell Signaling, #12936S, Granzyme B (E5V2L) Rabbit mAb (Cell signaling, #44153) and SHMT2 (E7F4Q) Rabbit mAb (Cell signaling, #3344), vinculin Mouse mAb (Sigma-aldrich, #V9131), actin (clone C4) Mouse mAb (MP biomedicals, #691001) antibodies diluted in 5% skimmed milk in Tris-Buffered NaCl Solution with Tween 20 (TBST)
Dihexa Catalogue number: HY-16969-50mg Supplier: Size: 50 mg _$$_ Product is available in: 918.00 Shipping is calculated in checkout This product is for research use only and is not for human consumption or therapeutic use
General Instructions Here are the instructions that are required to be kept in mind while using this face wash: Use Glutathione 4% & Glycolic Acid 6% Face Wash Gel twice a day (AM-PM) for better and fast results
designed the protocol and supervised the work while Okoroafor C.H
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