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S4b) and transfected these constructed vectors into HEK293 cells along with synthetic mature miR-483-3p double-stranded RNA (miR-483-3p mimic, miR-483-3p) or a control miRNA (miR-C) with a scrambled sequence

Following subcutaneous injection, the peptide enters systemic circulation with bioavailability of 14-19% in rats, while intramuscular administration achieves 45-51% bioavailability in beagle dogs, indicating both route-dependent and species-dependent absorption characteristics

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Both render the solution unsafe or ineffective

Rather than acting through a single mechanism, this peptide appears to coordinate multiple healing processes simultaneously, which may explain its consistent effectiveness across different wound types and tissue locations