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The sections were then incubated with primary antibodies against occludin (Abcam, dilution 1:100) and ZO-1 (ThermoFisher, dilution 1:100), diluted in PBS containing 0.2% BSA and 0.1% saponin at 4 C overnight, washed, and incubated for 1 h with Alexa Fluor 647 donkey anti-rabbit IgG secondary antibody (Invitrogen, dilution 1:400)

Rare Side Effects In addition to common side effects, there are rare instances where individuals may experience more severe reactions: Asthma Symptoms : Those with asthma may experience exacerbated symptoms, such as wheezing or shortness of breath

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Ozempic is approved to treat type 2 diabetes Wegovy is specifically approved for chronic weight management In some cases, providers may prescribe Ozempic off-label for weight management when appropriate, but Wegovy is the formulation designed and approved for weight loss

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Hakim RB, Gray RH, Zacur H