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Effectiveness, safety, and major adverse limb events in atrial fibrillation patients with concomitant diabetes mellitus treated with non-vitamin K antagonist oral anticoagulants

Various structural alterations were made at the N-terminal such as substitution of D-norleucine for L-norleucine, N-acetylation of norleucine, and replacement of norleucine with GABA in order to improve overall stability by reducing susceptibility to aminopeptidases

doi: 10.1111/ALL.15977 209 WilkinsonMHartAMilanSJSugumarK

PMID 12654482

Washed beads were eluted in 20 l 0.2% SDS in 50 mM HEPES with 4 Laemmli sample buffer (Bio-Rad, 1610747) and boiled at 95 C for western blot analysis

This is the most critical area of change for practitioners and patients in 2026