Samples were then incubated with 30 l of freshly made MOX solution (40 mg methoxyamine hydrochloride dissolved in 1ml of pyridine) for 45 min at 45C to protect keto groups, followed by the incubation with 70 l of MTBSTFA (N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide) for 45 min at 45C to derivatize metabolites
If the pen was in a hot car, on a sunny windowsill, or in a room without air conditioning during summer, the situation is more concerning
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Biotinylated GPX4 protein prepared by Thermo EZ-Link long-chain biotinylation reagent was connected to a Super Streptavidin (SSA) biosensor by immersing the sensor in a 96-well black plate loaded with protein solution, the concentration of which was predetermined by preliminary control experiments to achieve the best signal-to-noise ratio
10.1523/JNEUROSCI.6569-10.2011 J
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