Commonly used reducing agents include TCEP, DTT, and bME
A great storage environment can't protect against poor aseptic technique
This discrepancy may be explained by the comparatively low photosynthetic capacity of young leaves and by the induction of photo-protective mechanisms (non-photochemical quenching, photorespiration) (Hipsch et al
Frost-free freezers use small, periodic heating cycles to prevent ice buildup, and these temperature fluctuations can degrade sensitive peptides over time
Images were taken at 63 magnification using a Zeiss LSM510 confocal microscope or at 40 or 60 magnification using a Nikon A1 inverted confocal with a spectral detector or a Nikon Inverted spinning disk confocal microscope and at 100 with a Nikon N-SIM-S Super Resolution Microscope
Supporting the liver may be daunting at first, but believe me, it is well worth it