[DOI] [PubMed] [Google Scholar] 138.Nemeth E, Tuttle MS, Powelson J, et al
Performance benchmarks should include third-party test reports for efficacy claims like collagen production stimulation
However, as research has progressed, Erastins induction of cell death is not limited solely to ferroptosis but may involve the combined action of multiple cell death pathways (13, 14)
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Immunoblot analysis For detection of Gclc (Santa Cruz Biotechnology sc-390811), Pdha-1 (Abcam ab168379), DRP1 (Abcam ab184247), eIF4E (Abcam ab33766), p-eIF4E (Abcam ab76256), 4E-BP1 (Cell signaling 9452), p-4E-BP1 (Cell signaling 2855), DRP1 (Abcam ab184247), mTOR (Cell Signaling 2972), p-mTOR (Ser2448) (Cell Signaling 2971), p70 S6 (Cell Signaling 9202), p-p70 S6 (Thr421/Ser424) (Cell Signaling 9204), 15 10 6 cells were lysed with lysis buffer (CST 9803S) and protein/phosphatases inhibitors as per manufacturers protocol and blotted as described 44
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