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doi: 10.1016/j.biopsych.2015.07.028 199 LouZ.-Y.YuW.-B.ChenJ.LiL.JiangL.-S.XiaoB.-G.et al

This enzyme converts ATP to cyclic AMP (cAMP), a crucial second messenger

90-day old hCSs were dissociated using Primary Neuron Isolation Enzyme with papain (Pierce/Thermo Fisher Scientific/Fisher Scientific, Cat# PI88285) and plated at 250,000 cells/well onto the coated MEA plates