2018;13:e0198626
Research goals: Skin & Hair Description Glow Blend is a three-component peptide combination of GHK-Cu (the copper-binding tripeptide glycyl-L-histidyl-L-lysine, complexed with copper), BPC-157 (the synthetic stable gastric pentadecapeptide derived from human Body Protection Compound), and TB-500 (the N-acetylated 1723 fragment of thymosin beta-4)
There may also be interactions with other amino acids such as tyrosine, methionine, tryptophan and histidine, the only other amino acids that can be oxidized, or in the case of arginine, act as an anchorage site for Cu 1+ (Prudent and Girault, 2009)
Oxidized Prdx2 forms an interchain disulfide, which is recycled by thioredoxin and thioredoxin reductase, and this requires reducing equivalents from NADPH
For an 8-week protocol, that is typically 2 vials of KLOW, 1 bottle of bacteriostatic water, 56 syringes, and 112 swabs
Biotinylated goat anti-mouse IgG or goat anti-rabbit IgG (Vector Labs, BA-9200 1:200 and BA-1000 1:200, respectively) secondary antibodies were added in blocking buffer and incubated for 1 h at room temperature
