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The supernatant was removed from the pellet and stored at -80C or processed immediately for gel electrophoresis

TIming to full effects: 3-6 months

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Non-CG methylation might have an important role in the maintenance of pluripotency, as it re-appears in induced pluripotent cells and is abundant in gene bodies of highly expressed genes (Lister et al., 2009)

[DOI] [PubMed] [Google Scholar] Ernst A., Alkass K., Bernard S., Salehpour M., Perl S., Tisdale J., Possnert G., Druid H., Frisen J

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