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The study was designed using three animals per stage and was meticulously observed for any signs of toxicity or death

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The knockout of antioxidant-defense-system-related genes (SOD1, SOD2, NRF2) in animal models typically results in progressive neuro- and retinal degeneration, with loss of RGCs, decreases in ERG waves, and increases in cleaved-caspase-3-positive apoptotic cells in the retina and varying degrees of disease progression with age [227,228,406]

(7) Virmani MA, et al