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cDNA was diluted by five times and 1 l was used to do first round PCR with 3RACE-R1, Renilla-F1, and Firefly-F1 primers in one reaction

For secretory recombinant proteins produced in yeast, high mannose hyperglycosylation may be a major issue, as it may potentially alter functional properties of the proteins 27

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Its short-acting and typically dosed daily when used

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