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Cell 118:675685 Taherzadeh MJ (1999) Ethanol from lignocellulose: physiological effects of inhibitors and fermentation strategies

Reconstitution introduces: a new pH a new ionic environment a different solvent system If that shift pushes the peptide outside its stability window: charge drops repulsion weakens molecules begin to aggregate This is often described as a solubility cliff The BAC effect Bacteriostatic water is not neutral

We examined the use of the NBzl photosensitive PG under exposure to our conditions because of its relatively easy incorporation to the Cys side chains and its high stability in SPPS 20,21

Store lyophilized powder at -20C for long-term storage

The latter possibility is consistent with the report that KLF9 acts as a transcriptional mediator of glucocorticoid-regulated, KLF9 family member KLF2 in macrophages to promote inflammation (Chinenov et al., 2014)