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Notably, 50% of measured NADP + in frozen blood originated from NADPH, allowing for extrapolation of NADPH and NADP + levels before freezing, if fresh sample analysis is not feasible

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An iScript cDNA synthesis kit (Bio-Rad, Hercules, CA) was used to amplify 1 g of RNA per reaction in a MyCycler thermal cycler (Bio-Rad)

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