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Covalent modification of proteins To assess the extent of covalent modification of KRAS G13C 1169 , 50 M Ras protein was incubated with a 10-fold molar excess of acryl-bearing nucleotides in a buffer containing 100 mM HEPES (pH 7, 7.5 and 8) or 100 mM CHES (pH 8.5, 9 and 9.5), 50 mM NaCl, 1 mM TCEP, 5% glycerol and 1 mM EDTA

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