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Wash 2 times and drain to obtain Fmoc-Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu- Val-Wang Resin

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These results indicated that intracellular ATG2 protein exists as an S-palmitoylated entity

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Standardized SOPs with specified reconstitution volume and aseptic technique prevent all three failures