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Finally, the non-adherent cells were centrifuged again, and the cell pellet was resuspended in 10 mL of culture medium (Hams F-12 medium containing 1x GlutaMAX, 15 mM HEPES, 0.8 mM CaCl, 0.25% BSA, Insulin-transferrin-selenium (ITS, Sigma, I3146), 2% FBS, and 1% P/S)

J Leukoc Biol 1999

Golba B, Zhong Z, Romio M, Almey R, Deforce D, Dhaenens M, Sanders NN, Benetti EM

Statements Author contributions YZ: Data curation, Methodology, Investigation, Conceptualization, Visualization, Writing original draft

Thiols (R-SH), also known as mercaptans, are widely used in the synthesis of pharmaceuticals and agrochemicals due to their reactivity and versatility [9]