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The preparations were subjected to additional incubation with a 1:10000 dilution of an anti-immunoglobin horseradish peroxidase-linked antibody (Beijing Zhongshan Jinqiao Biological Technology Co., Beijing, China) at room temperature for 1 hr

For example, the hippocampus, crucial for memory formation, often shows significant atrophy in Alzheimers disease, he noted

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