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The application of these inclusion criteria has demonstrated that a large proportion of the study population presented with inadequate Zn intake levels for their ages: of the 804 participants, the study population decreased to 254 participants when intake levels were taken into account, a 68.41% reduction of the populations size, suggesting that correcting for possible distorting factors may be necessary for future studies, although this also entails reducing the sample size substantially
The study found that cigarette smoke had a negative effect on ovarian function and the effect was dependent on the amount smoked and how long the subject had been a smoker (7)
