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The cells were maintained in RPMI-1640 medium supplemented with 10% FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin (Life Technologies/Gibco, Oshima, New York), in an incubator at 37 C and 5% CO 2

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Chuck Woolery , who rose to fame as a host on popular gameshows including Wheel of Fortune and The Dating Game , died on Nov