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Following endopeptidase digestion of the isolated proteins, the peptide fragments were analysed by direct nano-flow LCMS/MS (liquid chromatographytandem mass spectrometry) 17
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Sherry N, Hagopian W, Ludvigsson J, et al
A tumours metabolic requirements and their regulation can be changed upon cell adaptation to in vitro culture conditions, where the concentrations of glucose and glutamine are significantly higher than physiological, whereas other components are quite often missing
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